Necropsy protocol for newborn mice

Virulence in Newborn Mice

A miniature mechanical ventilator for newborn mice.

Transgenic/knockout mice with pre-defined mutations have become increasingly popular in biomedical research as models of human diseases. In some instances, the resulting mutation may cause cardiorespiratory distress in the neonatal or adult animals and may necessitate resuscitation. Here we describe the design and testing of a miniature and versatile ventilator that can deliver varying ventilat...

A Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice

Background: The purification of Schwann cells has proven to be a difficult process, with most methods requiring the use of special equipment. However, obtaining a sufficient number and high purity of Schwann cells is an integral aspect in their use for clinical application. Therefore, the aim of this study was to establish a simple and effective protocol for the isolation and purification of Sc...

Chylous Ascites in Newborn Mice.

In I958, during breeding experiments in the Department of Genetics at Cambridge, a small -proportion of newbom offspring of backcross matings of the gene ragged (Ra, Slee, I957) developed a swollen belly. The affected animals appeared normal at birth but about 24 hours after they had begun to suckle their abdomens became swollen with an accumulation of milk-like fluid in the peritoneal cavity. ...

A standard protocol for blood pressure measurement in the newborn.

OBJECTIVES Improvements in neonatal care have resulted in increasing survival of extremely premature infants whose hospital course often runs into weeks or months. Some interventions during the acute care of these neonates, such as umbilical catheterization and use of steroids, not infrequently result in elevation of blood pressure (BP). It is, therefore, essential that these infants be monitor...